Workshop "Der Prinzipat als Paradoxie" im Bereich Alte Geschichte / Latinistik

  • History
    Braun, Theresa S.; Drescher, Malte; Summerer, Daniel (2019): Expanding the Genetic Code for Site-Directed Spin-Labeling International journal of molecular sciences. 2019, 20(2), 373. eISSN 1422-0067. Available under: doi: 10.3390/ijms20020373

Expanding the Genetic Code for Site-Directed Spin-Labeling


Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy enables studies of the structure, dynamics, and interactions of proteins in the noncrystalline state. The scope and analytical value of SDSL⁻EPR experiments crucially depends on the employed labeling strategy, with key aspects being labeling chemoselectivity and biocompatibility, as well as stability and spectroscopic properties of the resulting label. The use of genetically encoded noncanonical amino acids (ncAA) is an emerging strategy for SDSL that holds great promise for providing excellent chemoselectivity and potential for experiments in complex biological environments such as living cells. We here give a focused overview of recent advancements in this field and discuss their potentials and challenges for advancing SDSL⁻EPR studies.

Origin (projects)

Funding sources
Name Finanzierungstyp Kategorie Project no.
Sonstige Universität third-party funds research funding program 602/23
Further information
Period: 23.06.2023 – 31.12.2023